Journal
MOLECULAR BRAIN
Volume 7, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1756-6606-7-9
Keywords
mGlu1 alpha receptor; Lipid rafts; Caveolin; Calcium
Categories
Funding
- Korea Healthcare technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea [A102054, A110861, A120476]
- National Research Foundation of Korea (NRF) - Korea government (MEST) [2009-0080939, 2011-0030737]
- Korea Health Promotion Institute [A102054, A110861] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2009-0080939] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Background: Group I metabotropic glutamate receptors (mGlu1/5 receptors) have important roles in synaptic activity in the central nervous system. They modulate neuronal excitability by mobilizing intracellular Ca2+ following receptor activation. Also, accumulating evidence has indicated the association of Ca2+ signaling with lipid rafts. Caveolin, an adaptor protein found in a specialized subset of lipid rafts, has been reported to promote the localization of membrane proteins to lipid rafts. Results: In the present study, we investigated the role of lipid rafts on the mGlu1 alpha receptor-mediated Ca2+ signaling in association with caveolin in hippocampal primary neurons and HEK293 cells. We show that the disruption of lipid rafts using methyl-beta-cyclodextrin markedly decreased mGlu1 alpha receptor-mediated Ca2+ transients and lipid rafts localization of the receptor. Furthermore, transfection of mGlu1 alpha receptor with mutated caveolin-binding domain reduced localization of the receptor to lipid rafts. Also, application of a peptide blocker of mGlu1 alpha receptor and caveolin binding reduced the Ca2+ signaling and the lipid rafts localization. Conclusions: Taken together, these results suggest that the binding of mGlu1 alpha receptor to caveolin is crucial for its lipid rafts localization and mGlu1 alpha receptor-mediated Ca2+ transients.
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