Comparative Evaluation of Conventional and Nanosilver-Containing Leucocyte and Platelet-Rich Fibrin/Biomaterial in the Anti-Biofilm Formation of Standard Species of Candida and Streptococcus

Background: Biomaterial products like Leukocyte and platelet-rich fibrin (L-PRF) membranes can be used in a variety of medical fields to increase the anti-infective defense system and promote tissue regeneration and wound healing due to platelet growth factors. It is one of the oldest approaches of regenerative tissue. In addition, antifungal and bacterial activities against some microorganisms are reported in the literature for silver nanoparticles. Objectives: The aim of this study was to evaluate the anti-biofilm formation of L-PRF and its modification with silver nanoparticles (AgNPL-PRF) against microorganisms-associated human health conditions. Methods: Blood samples were obtained and L-PRF and AgNPL-PRF membranes were produced using blood centrifugation. The biofilm formation was evaluated by incubating the standard Candida and Streptococcus species with the membranes. The incubated membranes were rinsed to remove unattached cells and incubated in an RPMI 1640 medium. The populations of growing microorganisms in the medium were evaluated by ELISA reader. Results: The difference in the presence of microorganisms in RPMI medium demonstrated a statistically significant inhibition of biofilm formation of Candida albicans and C. parapsilosis while it was insignificant in C. glabrata and Streptococcus mitis in both types of membranes. Conclusions: Silver nanoparticles-treated L-PRF as a biological material presented the more inhibition of biofilm formation of contaminating microorganisms and it can be used as anti-infectious material in the surgical sites.