Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 104, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/52581
Keywords
Developmental Biology; Issue 104; Animal model; physiology; development; anesthesia; surgery; ECG; chronic experimentation; multivariate data acquisition; inflammation; neuroscience
Categories
Funding
- Molly Towell Perinatal Research Foundation
- Canadian Institutes of Health Research (CIHR)
- Fonds de Recherche du Quebec-Sante (FRQS)
- CIHR-Quebec Training Network in Perinatal Research (QTNPR)
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The chronically instrumented pregnant sheep has been used as a model of human fetal development and responses to pathophysiologic stimuli such as endotoxins, bacteria, umbilical cord occlusions, hypoxia and various pharmacological treatments. The life-saving clinical practices of glucocorticoid treatment in fetuses at risk of premature birth and the therapeutic hypothermia have been developed in this model. This is due to the unique amenability of the non-anesthetized fetal sheep to the surgical placement and maintenance of catheters and electrodes, allowing repetitive blood sampling, substance injection, recording of bioelectrical activity, application of electric stimulation and in vivo organ imaging. Here we describe the surgical instrumentation procedure required to achieve a stable chronically instrumented non-anesthetized fetal sheep model including characterization of the post-operative recovery from blood gas, metabolic and inflammation standpoints.
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