Journal
HUMAN VACCINES & IMMUNOTHERAPEUTICS
Volume 10, Issue 5, Pages 1306-1308Publisher
LANDES BIOSCIENCE
DOI: 10.4161/hv.28077
Keywords
DNA vaccination; nonviral gene therapy; pneumatic delivery; supercoil; topoisoforms; vaccine; vector safety
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While DNA vaccination using plasmid vectors is highly attractive, there is a need for further vector optimization regarding safety, stability, and efficiency. In this commentary, we review the minicircle vector (MC), which is an entity devoid of plasmid bacterial sequences, as an alternative to the traditional plasmid construct. The commentary highlights the recent discovery by Stenler et al. (2014) that the small size of an MC enables improved resistance to the shearing forces associated with e.g. pneumatic delivery methods. This observation may have implications for the regulatory agencies' requirement of plasmid integrity and quality.
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