Journal
GENOME MEDICINE
Volume 10, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s13073-018-0572-z
Keywords
Antibiotic resistance; Resistance mutations; Deep sequencing; Staphylococcus aureus; Mycobacterium tuberculosis; Rifampicin; Daptomycin
Categories
Funding
- National Health and Medical Research Council (NHMRC), Australia [GNT1066791, GNT1008549, GNT1105905]
- Department of Microbiology and Immunology at The University of Melbourne
Ask authors/readers for more resources
Mutation acquisition is a major mechanism of bacterial antibiotic resistance that remains insufficiently characterised. Here we present RM-seq, a new amplicon-based deep sequencing workflow based on a molecular barcoding technique adapted from Low Error Amplicon sequencing (LEA-seq). RM-seq allows detection and functional assessment of mutational resistance at high throughput from mixed bacterial populations. The sensitive detection of very low-frequency resistant sub-populations permits characterisation of antibiotic-linked mutational repertoires in vitro and detection of rare resistant populations during infections. Accurate quantification of resistance mutations enables phenotypic screening of mutations conferring pleiotropic phenotypes such as in vivo persistence, collateral sensitivity or cross-resistance. RM-seq will facilitate comprehensive detection, characterisation and surveillance of resistant bacterial populations (https://github.com/rguerillot/RM-seq).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available