Journal
G3-GENES GENOMES GENETICS
Volume 4, Issue 7, Pages 1291-1295Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.114.012179
Keywords
Cas9 protein; RGEN; Drosophila
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Funding
- MSIP (Korea) [NRF-2011-0023507]
- KRIBB initiative program (Korea)
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We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
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