Journal
G3-GENES GENOMES GENETICS
Volume 3, Issue 1, Pages 1-4Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.112.004481
Keywords
C(2) EN; long chromosome; HisH2Av-mRFP1; neuroblast; mitosis
Categories
Funding
- National Institutes of Health [GM046409-19]
- California Institute for Regenerative Medicine [TG2-01157, FA1-00617-1]
- W. M. Keck Foundation [CS-11-99]
- Fundacao para a Ciencia e Tecnologia
- Fundacao para a Ciencia e Tecnologia [PTDC/BIA-BCM/100305/2008]
- UC Santa Cruz Open Access Fund
- Fundação para a Ciência e a Tecnologia [PTDC/BIA-BCM/100305/2008] Funding Source: FCT
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Among all organisms, Drosophila melanogaster has the most extensive well-characterized collection of large-scale chromosome rearrangements. Compound chromosomes, rearrangements in which homologous chromosome arms share a centromere, have proven especially useful in genetic-based surveys of the entire genome. However, their potential has not been fully realized because compound autosome stocks are refractile to standard genetic manipulations: if outcrossed, they yield inviable aneuploid progeny. Here we describe two strategies, cold-shock and use of the bubR1 mutant alleles, to produce nullo gametes through nondisjunction. These gametes are complementary to the compound chromosome-bearing gametes and thus produce viable progeny. Using these techniques, we created a compound chromosome two C(2) EN stock bearing a red fluorescent protein-histone transgene, facilitating live analysis of these unusually long chromosomes.
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