Journal
CELL REPORTS
Volume 8, Issue 1, Pages 75-83Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2014.05.053
Keywords
-
Categories
Funding
- La Ligue nationale contre le cancer
- Labex Memo Life
- National Institute of General Medical Sciences of the National Institutes of Health [R25GM105608]
- Region Ile-de-France under C'Nano IdF (Center of Competences in NanoSciences for the Paris region)
- Fondation pour la Recherche Medicale (FRM)
- Institut Pasteur
- [ANR-10-BLAN-1222]
- [ANR-10-LABX-54]
- [MEMO LIFE/ANR-11-IDEX-0001-02]
Ask authors/readers for more resources
Transcription is a stochastic process occurring mostly in episodic bursts. Although the local chromatin environment is known to influence the bursting behavior on long timescales, the impact of transcription factors (TFs)-especially in rapidly inducible systems-is largely unknown. Using fluorescence in situ hybridization and computational models, we quantified the transcriptional activity of the protooncogene c-Fos with single mRNA accuracy at individual endogenous alleles. We showed that, during MAPK induction, the TF concentration modulates the burst frequency of c-Fos, whereas other bursting parameters remain mostly unchanged. By using synthetic TFs with TALE DNA-binding domains, we systematically altered different aspects of these bursts. Specifically, we linked the polymerase initiation frequency to the strength of the transactivation domain and the burst duration to the TF lifetime on the promoter. Our results show how TFs and promoter binding domains collectively act to regulate different bursting parameters, offering a vast, evolutionarily tunable regulatory range for individual genes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available