Journal
CELL REPORTS
Volume 5, Issue 1, Pages 259-270Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2013.08.039
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Funding
- Singapore A*STAR National Science Scholarship
- NIH [DK-068348, R24 DK-090963, R01GM-089903, P30-ES002109]
- National Science Foundation [DB1-0821391]
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Diet-induced obesity (DIO) predisposes individuals to insulin resistance, and adipose tissue has a major role in the disease. Insulin resistance can be induced in cultured adipocytes by a variety of treatments, but what aspects of the in vivo responses are captured by these models remains unknown. We use global RNA sequencing to investigate changes induced by TNF-alpha, hypoxia, dexamethasone, high insulin, and a combination of TNF-alpha and hypoxia, comparing the results to the changes in white adipose tissue from DIO mice. We found that different in vitro models capture distinct features of DIO adipose insulin resistance, and a combined treatment of TNF-alpha and hypoxia is most able to mimic the in vivo changes. Using genome-wide DNase I hypersensitivity followed by sequencing, we further examined the transcriptional regulation of TNF-alpha-induced insulin resistance, and we found that C/EPB beta is a potential key regulator of adipose insulin resistance.
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