Journal
CELL REPORTS
Volume 3, Issue 4, Pages 978-987Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2013.03.033
Keywords
-
Categories
Funding
- Ministry of Education, Culture, Sports, Science & Technology in Japan
- Japan Society for the Promotion of Science (KIBAN)
- Japan Society for the Promotion of Science (WAKATE)
- Japanese Ministry of Health, Labour and Welfare
- Global COE Program
- Astellas Foundation for Research on Metabolic Disorders
- Kowa Life Science Foundation
- Shimadzu Science Foundation
- Takeda Foundation
- Toray Science Foundation
- Yamada Science Foundation
- Grants-in-Aid for Scientific Research [23680040, 221S0003, 21220006, 25000015, 11J04427, 20670002] Funding Source: KAKEN
Ask authors/readers for more resources
How information encoded in glutamate release rates at individual synapses is converted into biochemical activation patterns of postsynaptic enzymes remains unexplored. To address this, we developed a dual fluorescence resonance energy transfer (FRET) imaging platform and recorded CaMKII alpha and calcineurin activities in hippocampal neurons while varying glutamate uncaging frequencies. With little spine morphological change, 5 Hz spine glutamate uncaging strongly stimulated calcineurin, but not CaMKII alpha. In contrast, 20 Hz spine glutamate uncaging, which induced spine growth, activated both CaMKII alpha and calcineurin with distinct spatiotemporal kinetics. Higher temporal resolution recording in the soma revealed that CaMKII alpha activity summed supralinearly and sensed both higher frequency and input number, thus acting as an input frequency/number decoder. In contrast, calcineurin activity summated sublinearly with increasing input number and showed little frequency dependence, thus functioning as an input number counter. These results provide evidence that CaMKII alpha and calcineurin are fine-tuned to unique bandwidths and compute input variables in an asymmetric manner.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available