Journal
BMC PULMONARY MEDICINE
Volume 10, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1471-2466-10-30
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Funding
- Flight Attendant Medical Research Institute (FAMRI)
- Sepracor, Inc.
- National Institutes of Health (NIH) [RO1 HL088264]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL088264] Funding Source: NIH RePORTER
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Background: Airway epithelial cells are critical in host defense against bacteria including Mycoplasma pneumoniae (Mp) in chronic obstructive pulmonary disease (COPD) and asthma. beta 2-agonists are mainstay of COPD and asthma therapy, but whether beta 2-agonists directly affect airway epithelial host defense functions is unclear. Methods: Epithelial cells from bronchial brushings of normal (n = 8), asthma (n = 8) and COPD (n = 8) subjects were grown in air-liquid interface cultures, and treated with cigarette smoke extract (CSE) and/or Th2 cytokine IL-13, followed by Mp infection and treatment with beta 2-agonists albuterol and formoterol for up to seven days. Mp and host defense proteins short palate, lung, and nasal epithelial clone 1 (SPLUNC1) and beta-defensin-2 were quantified. Expression of beta 2-adrenergic receptors was also measured by real-time quantitative RT-PCR. Results: (R)- or racemic albuterol and (R,R)- or racemic formoterol significantly decreased Mp levels in normal and asthma epithelial cells. Normal cells treated with Mp and (R)- or racemic albuterol showed an increase in SPLUNC1, but not in beta-defensin-2. COPD cells did not respond to drug treatment with a significant decrease in Mp or an increase in SPLUNC1. IL-13 attenuated drug effects on Mp, and markedly decreased SPLUNC1 and beta 2-adrenergic receptors. Conclusions: These results for the first time show that beta 2-agonists enhance host defense functions of primary bronchial epithelial cells from normal and asthma subjects, which is attenuated by IL-13.
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