4.5 Article

Analysis of the human intestinal microbiota from 92 volunteers after ingestion of identical meals

Journal

BENEFICIAL MICROBES
Volume 4, Issue 2, Pages 187-193

Publisher

WAGENINGEN ACADEMIC PUBLISHERS
DOI: 10.3920/BM2012.0045

Keywords

human intestinal microbiota; identical meals; T-RFLP

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The intestinal microbiota composition of 92 volunteers living in Japan was identified following the consumption of 'identical meals' (1,879 kcal/day) for 3 days. When faecal samples were analysed by terminal restriction fragment length polymorphism with several primer-restriction enzyme systems and then clustered, the patterns could be divided into 2 clusters. Contribution tests and partition modelling showed that OTU211 of the 35f-MspI system and OTU237 of the 35f-AluI system were key factors in the distribution of these groups. However, significant differences among these groups in terms of body mass index and age were not observed.

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