Journal
ADVANCED HEALTHCARE MATERIALS
Volume 2, Issue 12, Pages 1600-1605Publisher
WILEY
DOI: 10.1002/adhm.201300135
Keywords
AIE dots; cancer metastasis; cell tracing; dual-modality; fluorescence imaging; gadolinium; magnetic resonance imaging
Funding
- Institute of Materials Research and Engineering of Singapore [IMRE/12-8P1103, IMRE/13-8P1104]
- Singapore National Research Foundation [R-279-000-390-281]
- Research Grants Council of Hong Kong [603509, HKUST2/CRF/10, 604711, N_HKUST620/11]
- National Science Foundation of China [20974028]
- Guangdong Innovative Research Team Program [201101C0105067115]
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Understanding the localization and engraftment of tumor cells at postintravasation stage of metastasis is of high importance in cancer diagnosis and treatment. Advanced fluorescent probes and facile methodologies for cell tracing play a key role in metastasis studies. In this work, we design and synthesize a dual-modality imaging dots with both optical and magnetic contrast through integration of a magnetic resonance imaging reagent, gadolinium(III), into a novel long-term cell tracing probe with aggregation-induced emission (AIE) in far-red/near-infrared region. The obtained fluorescent-magnetic AIE dots have both high fluorescence quantum yield (25%) and T-1 relaxivity (7.91 mM(-1) s(-1)) in aqueous suspension. After further conjugation with a cell membrane penetrating peptide, the dual-modality dots can be efficiently internalized into living cells. The gadolinium(III) allows accurate quantification of biodistribution of cancer cells via intraveneous injection, while the high fluorescence provides engraftment information of cells at single cellular level. The dual-modality AIE dots show obvious synergistic advantages over either single imaging modality and hold great promises in advanced biomedical studies.
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