Journal
ADVANCED HEALTHCARE MATERIALS
Volume 3, Issue 1, Pages 115-125Publisher
WILEY-BLACKWELL
DOI: 10.1002/adhm.201300126
Keywords
adipose-derived stem cells; bioactive glass; mesenchymal stem cells; osteogenic differentiation; submicron particles
Funding
- EPSRC [EP/E057098/1]
- European Union [257182]
- ERC grant Naturale
- Engineering and Physical Sciences Research Council [EP/E057098/1, EP/I020861/1] Funding Source: researchfish
- EPSRC [EP/I020861/1] Funding Source: UKRI
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Spherical monodispersed bioactive particles are potential candidates for nanocomposite synthesis or as injectable particles that could be internalized by cells for the local sustained delivery of inorganic therapeutic ions (e.g., calcium or strontium). Particles are also likely to be released from porous bioactive glass and sol-gel hybrid scaffolds as they degrade; thus, it is vital to investigate their interaction with cells. Spherical monodispersed bioactive glass particles (mono-SMBG), with diameters of 215 +/- 20 nm are synthesized using a modified Stober process. Confocal and transmission electron microscopy demonstrate that mono-SMBGs are internalized by human bone marrow (MSCs) and adipose-derived stem cells (ADSCs) and located within cell vesicles and in the cytoplasm. Particle dissolution inside the cells is observed. Alamar Blue, MTT and Cyquant assays demonstrate that 50 g mL(-1) of mono-SMBGs did not inhibit significantly MSC or ADSC metabolic activity. However, at higher concentrations (100 and 200 g mL(-1)) small decrease in metabolic activity and total DNA is observed. Mono-SMBG did not induce ALPase activity, an early marker of osteogenic differentiation, without osteogenic supplements; however, in their presence osteogenic differentiation is achieved. Additionally, large numbers of particles are internalized by the cells but have little effect on cell behavior.
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