Journal
ADVANCED HEALTHCARE MATERIALS
Volume 3, Issue 3, Pages 412-423Publisher
WILEY
DOI: 10.1002/adhm.201300033
Keywords
carbon nanotubes; cardiac biomarkers; troponin; fluorescence; near-infrared
Funding
- National Science Foundation
- Novartis
- Army Research Office
- MIT
- Deutsche Forschungsgemeinschaft
- Department of Energy SCGF [DE-AC05-06OR23100]
- Damon Runyon Cancer Research Foundation (DFS) [2050-10]
- Memorial Sloan-Kettering Cancer Center
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Patients with chest pain account for 10% of US emergency room visits according to data from the Center for Disease Control and Prevention (2013). For triage of these patients, cardiac biomarkers troponin I and T are endorsed as standard indicators for acute myocardial infarction (AMI, or heart attack). Thus, there is significant interest in developing a rapid, point-of-care (POC) device for troponin detection. In this work, a rapid, quantitative, and label-free assay, which is specific for cardiac troponin T (cTnT) detection, using fluorescent single-walled carbon nanotubes (SWCNTs), is demonstrated. Chitosan-wrapped carbon nanotubes are cross-linked to form a thin gel that is further functionalized with nitrilotriacetic acid (NTA) moieties. Upon chelation of Ni2+, the Ni2+-NTA group binds to a hexa-histidine-modified troponin antibody, which specifically recognizes the target protein, troponin T. As the troponin T binds to the antibody, the local environment of the sensor changes, allowing direct troponin detection through intensity changes in SWCNT bandgap fluorescence. This platform represents the first near-infrared SWCNT sensor array for cTnT detection. Detection can be completed within 5 min, demonstrating a linear response to cTnT concentration and an experimental detection limit of 100 ng mL(-1) (2.5 nm). This platform provides a promising new tool for POC AMI detection in the future. Moreover, the work presents two new methods of quantifying the number of amines and carboxylic groups, respectively, in a carbon hydrogel matrices.
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