Journal
ACS SYNTHETIC BIOLOGY
Volume 7, Issue 9, Pages 2029-2036Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00167
Keywords
formate assimilation; one-carbon metabolism; glycine cleavage complex; directed evolution; long-term continuous culture; synthetic metabolism
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Funding
- Commissariat a l'Energie Atomique (CEA), France
- European Union [763911]
- H2020 Societal Challenges Programme [763911] Funding Source: H2020 Societal Challenges Programme
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Endowing biotechnological platform organisms with new carbon assimilation pathways is a key challenge for industrial biotechnology. Here we report progress toward the construction of formatotrophic Escherichia coli strains. Glycine and serine, universal precursors of one-carbon compounds oxidized during heterotrophic growth, are produced from formate and CO2 through a reductive route. An adaptive evolution strategy was applied to optimize the enzymatic steps of this route in appropriate selection strains. Metabolic labeling experiments with C-13-formate confirm the redirected carbon-flow. These results demonstrate the high plasticity of the central carbon metabolism of E. coli and the applicative potential of directed evolution for implementing synthetic pathways in microorganisms.
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