Journal
ACS SYNTHETIC BIOLOGY
Volume 4, Issue 10, Pages 1070-1076Publisher
AMER CHEMICAL SOC
DOI: 10.1021/sb500299c
Keywords
directed evolution; transcription; genetic circuits
Categories
Funding
- Welch Foundation [F-1654]
- National Security Science and Engineering Faculty Fellowship [FA9550-10-1-0169]
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T7 RNA polymerase is the foundation of synthetic biological circuitry both in vivo and in vitro due to its robust and specific control of transcription from its cognate promoter. Here we present the directed evolution of a panel of orthogonal T7 RNA polymerase:promoter pairs that each specifically recognizes a synthetic promoter. These newly described pairs can be used to independently control up to six circuits in parallel.
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