Journal
ACS SYNTHETIC BIOLOGY
Volume 4, Issue 1, Pages 32-38Publisher
AMER CHEMICAL SOC
DOI: 10.1021/sb500165g
Keywords
continuous culture; turbidostat; characterization; fluorescence detection
Categories
Funding
- National Science Foundation (NSF) [1317653]
- Paul G. Allen Family Foundation
- NSF [1120425]
- National Institute of General Medical Sciences from the National Institutes of Health [P41 GM103533]
- [T32 HG00035]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [T32HG000035] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103533] Funding Source: NIH RePORTER
- Direct For Biological Sciences [1120425] Funding Source: National Science Foundation
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Engineered biological circuits are often disturbed by a variety of environmental factors. In batch culture, where the majority of synthetic circuit characterization occurs, environmental conditions vary as the culture matures. Turbidostats are powerful characterization tools that provide static culture environments; however, they are often expensive, especially when purchased in custom configurations, and are difficult to design and construct in a lab. Here, we present a low cost, open source multiplexed turbidostat that can be manufactured and used with minimal experience in electrical or software engineering. We demonstrate the utility of this system to profile synthetic circuit behavior in S. cerevisiae. We also demonstrate the flexibility of the design by showing that a fluorometer can be easily integrated.
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