Journal
ACS SYNTHETIC BIOLOGY
Volume 2, Issue 11, Pages 629-634Publisher
AMER CHEMICAL SOC
DOI: 10.1021/sb400048b
Keywords
natural products; polyketide; heterologous expression; prenyltransferase
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Funding
- National Institutes of Health [1DP1GM106413]
- NIH [R21AI081235]
- NSF [CHE-1048804]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1048804] Funding Source: National Science Foundation
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Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains.
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