Journal
THERANOSTICS
Volume 3, Issue 4, Pages 239-248Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.5113
Keywords
Upconversion luminescence; nanoparticle; silica coating; Raman spectroscopy; bio-sensing; magnetic separation; surface plasmon resonance
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Funding
- German Research Foundation (DFG)
- Austrian Science Fund (FWF) [I 442] Funding Source: researchfish
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We describe the preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF4 upconverting luminescent nanoparticles for protein conjugation. Their core was coated with a silica shell which then was modified with a poly(ethylene glycol) spacer and N-hydroxysuccinimide ester groups. The nanoparticles were characterized by transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and dynamic light scattering. The N-hydroxysuccinimide ester functionalization renders them highly reactive towards amine nucleophiles (e. g., proteins). We show that such particles can be conjugated to proteins. The protein-reactive UCLNPs and their conjugates to streptavidin and bovine serum albumin display multicolor emissions upon 980-nm continuous wave laser excitation. Surface plasmon resonance studies were carried out to prove bioconjugation and to compare the affinity of the particles for proteins immobilized on a thin gold film.
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