Journal
THERANOSTICS
Volume 2, Issue 7, Pages 734-745Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.4290
Keywords
preantral follicle; invasion; reproductive toxicity; in vitro culture system; QDs-transferrin bioconjugate
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Funding
- National Natural Science Foundation of China [30900335]
- National Basic Research Program of China [2012CB85802]
- Scientific Research Starting Foundation for Returned Overseas Chinese Scholars, Ministry of Education, China
- Natural Science Foundation of Guangdong Province [2008078, S2011010001223]
- Basic Research of Shenzhen [JC201005280500A]
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The toxicity of QD has been extensively studied over the past decade. However, the potential toxicity of QDs impedes its use for clinical research. In this work, we established a preantral follicle in vitro culture system to investigate the effects of QD-Transferrin (QDs-Tf) bioconjugates on follicle development and oocyte maturation. The preantral follicles were cultured and exposed to CdTe/ZnTe QDs-Tf bioconjugates with various concentrations and the reproductive toxicity was assessed at different time points post-treatment. The invasion of QDs-Tf for oocytes was verified by laser scanning confocal microscope. Steroid production was evaluated by immunoassay. C-band Giemsa staining was performed to observe the chromosome abnormality of oocytes. The results showed that the QDs-Tf bioconjugates could permeate into granulosa cells and theca cells, but not into oocyte. There are no obvious changes of oocyte diameter, the mucification of cumulus-oocyte-complexes and the occurrence of aneulpoidy as compared with the control group. However, delay in the antrum formation and decrease in the ratio of oocytes with first polar body were observed in QDs-Tf-treated groups. The matured oocytes with first polar body decreased significantly by similar to 16% (from 79.6 +/- 10 % to 63 +/- 2.9 %) when the concentration of QDs-Tf bioconjugates exceeded 2.89 nmol.L-1 (P < 0.05). Our results implied that the CdTe/ZnTe QDs-Tf bioconjugates were reproductive toxic for follicle development, and thus also revealed that this in vitro culture system of preantral follicle is a highly sensitive tool for study on the reproductive toxicity of nanoparticles.
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