Journal
SCIENTIFIC REPORTS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-31510-6
Keywords
-
Categories
Funding
- Norwegian Research Council [196386]
- Norwegian Cancer Society [163359, 163361]
- Legatet til Henrik Homans Minde
- Anders Jahres fond til vitenskapens fremme
- Novo Nordisk Fonden
- University of Oslo
Ask authors/readers for more resources
Polarization of T cells towards the antigen presenting cell (APC) is critically important for appropriate activation and differentiation of the naive T cell. Here we used imaging flow cytometry (IFC) and show that the activation induced Lck and Itk adapter T cell specific adapter protein (TSAd), encoded by SH2D2A, modulates polarization of T cells towards the APC. Upon exposure to APC presenting the cognate antigen Id, Sh2d2a-/-CD4+T cells expressing Id-specific transgenic T cell receptor (TCR), displayed impaired polarization of F-actin and TCR to the immunological synapse (IS). Sh2d2a-/- T-cells that did polarize F-actin and TCR still displayed impaired polarization of PKC xi, PAR3 and the microtubule-organizing center (MTOC). In vitro differentiation of activated Sh2d2a-/-T cells was skewed towards an effector memory (Tem) rather than a central memory (Tcm) phenotype. A similar trend was observed for Id-specific TCR Sh2d2a-/-T cells stimulated with APC and cognate antigen. Taken together our data suggest that TSAd modulates differentiation of experienced T cells possibly through polarization of CD4+T cells towards the APC.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available