Journal
SCIENTIFIC REPORTS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-28511-w
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Funding
- Academy of Finland [277495]
- Sigrid Juselius Foundation
- Jane and Aatos Erkko Foundation
- Competitive State Research Financing of the Expert Responsibility Area of Tampere University Hospital
- Competitive State Research Financing of the Expert Responsibility area of Oulu University Hospital
- Tampere Tuberculosis Foundation
- Emil Aaltonen Foundation
- Foundation of the Finnish Anti-Tuberculosis Association
- City of Tampere Science Foundation
- Vaino and Laina Kivi Foundation
- Finnish Cultural Foundation
- Central Foundation
- Finnish Concordia Fund
- Orion Research Foundation sr
- University of Tampere Doctoral Programme in Biomedicine and Biotechnology
- Biocenter Finland
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Tuberculosis ranks as one of the world's deadliest infectious diseases causing more than a million casualties annually. IL10 inhibits the function of Th1 type cells, and IL10 deficiency has been associated with an improved resistance against Mycobacterium tuberculosis infection in a mouse model. Here, we utilized M. marinum infection in the zebrafish (Danio rerio) as a model for studying Il10 in the host response against mycobacteria. Unchallenged, nonsense il10(e46/e46) mutant zebrafish were fertile and phenotypically normal. Following a chronic mycobacterial infection, il10(e46/e46) mutants showed enhanced survival compared to the controls. This was associated with an increased expression of the Th cell marker cd4-1 and a shift towards a Th1 type immune response, which was demonstrated by the upregulated expression of tbx21 and ifng1, as well as the down-regulation of gata3. In addition, at 8 weeks post infection il10(e46/e46) mutant zebrafish had reduced expression levels of proinflammatory cytokines tnfb and il1b, presumably indicating slower progress of the infection. Altogether, our data show that Il10 can weaken the immune defense against M. marinum infection in zebrafish by restricting ifng1 response. Importantly, our findings support the relevance of M. marinum infection in zebrafish as a model for tuberculosis.
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