Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-17148-w
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Funding
- Competence Network of Heart Failure - Integrated Research and Treatment Center (IFB) of the Federal Ministry of Education and Research (BMBF)
- German Research Council (DFG) [HI 1789/2-1]
- German Research Foundation (DFG)
- University of Wuerzburg
- Grants-in-Aid for Scientific Research [15K21774] Funding Source: KAKEN
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Brown adipose tissue (BAT) is an attractive therapeutic target to combat diabetes and obesity due to its ability to increase glucose expenditure. In a genetic rat model (ZDF fa/fa) of type-2 diabetes and obesity, we aimed to investigate glucose utilization of BAT by F-18-FDG PET imaging. Male Zucker diabetic fatty (ZDF) and Male Zucker lean (ZL) control rats were studied at 13 weeks. Three weeks prior to imaging, ZDF rats were randomized into a no-restriction (ZDF-ND) and a mild calorie restriction (ZDF-CR) group. Dynamic F-18-FDG PET using a dedicated small animal PET system was performed under hyperinsulinemic-euglycemic clamp. F-18-FDG PET identified intense inter-scapular BAT glucose uptake in all ZL control rats, while no focally increased F-18-FDG uptake was detected in all ZDF-ND rats. Mild but significant improved BAT tracer uptake was identified after calorie restriction in diabetic rats (ZDF-CR). The weight of BAT tissue and fat deposits were significantly increased in ZDF-CR and ZDF-ND rats as compared to ZL controls, while UCP-1 and mitochondrial concentrations were significantly decreased. Whitening and severely impaired insulin-stimulated glucose uptake in BAT was confirmed in a rat model of type-2 diabetes. Additionally, calorie restriction partially restored the impaired BAT glucose uptake.
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