Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-08324-z
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Funding
- INCA grant
- Marie Sklodowska Curie Actions FP7
- Swedish Research Council
- Swedish Parkinsons foundation
- Crafoordska Stiftelsen
- Wellcome Trust [Wellcome 200848/Z/16/Z, Wellcome 100140]
- Alzheimer's Society, UK [317, AS-SF-16-003]
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A major hallmark of Parkinson's disease (PD) is the presence of Lewy bodies (LBs) in certain neuronal tissues. LBs are protein-rich inclusions, in which alpha-synuclein (alpha-syn) is the most abundant protein. Since these inclusions are not present in healthy individuals, despite the high concentration of alpha-syn in neurons, it is important to investigate whether natural control mechanisms are present to efficiently suppress alpha-syn aggregation. Here, we demonstrate that a CRISPR/Cas9-mediated knockout (KO) of a DnaJ protein, DNAJB6, in HEK293T cells expressing alpha-syn, causes a massive increase in alpha-syn aggregation. Upon DNAJB6 re-introduction into these DNAJB6-KO HEK293T-alpha-syn cells, aggregation is reduced to the level of the parental cells. We then show that the suppression of alpha-syn aggregation is dependent on the J-domain of DNAJB6, as the catalytically inactive protein, which carries the H31Q mutation, does not suppress aggregation, when re-introduced into DNAJB6-KO cells. We further demonstrate, that the suppression of alpha-syn aggregation is dependent on the molecular chaperone Hsp70, which is consistent with the well-known function of J-domains of transferring unfolded and misfolded proteins to Hsp70. These data identify a natural control strategy to suppress alpha-syn aggregation and suggest potential therapeutic approaches to prevent or treat PD and related disorders.
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