Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-10646-x
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Funding
- Karolinska Institutet
- Estonian Research Council [PUT618]
- Agency of Science, Technology and Research, Singapore (A*STAR)
- Swedish research Council (VR-Med)
- Swedish research Council (EuroNanoMedII)
- Swedish Society of Medical Research (SSMF)
- BBSRC [BB/M024393/1] Funding Source: UKRI
- MRC [MR/M007715/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M024393/1] Funding Source: researchfish
- Medical Research Council [MR/M007715/1] Funding Source: researchfish
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Extracellular vesicles (EVs) play a pivotal role in cell-to-cell communication and have been shown to take part in several physiological and pathological processes. EVs have traditionally been purified by ultracentrifugation (UC), however UC has limitations, including resulting in, operator-dependant yields, EV aggregation and altered EV morphology, and moreover is time consuming. Here we show that commercially available bind-elute size exclusion chromatography (BE-SEC) columns purify EVs with high yield (recovery similar to 80%) in a time-efficient manner compared to current methodologies. This technique is reproducible and scalable, and surface marker analysis by bead-based flow cytometry revealed highly similar expression signatures compared with UC-purified samples. Furthermore, uptake of eGFP labelled EVs in recipient cells was comparable between BE-SEC and UC samples. Hence, the BE-SEC based EV purification method represents an important methodological advance likely to facilitate robust and reproducible studies of EV biology and therapeutic application.
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