Journal
BIOLOGY OPEN
Volume 4, Issue 9, Pages 1143-1153Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.011957
Keywords
Flagella; Lipid rafts; Membrane lipids; Mass spectrometry; Sphingolipids; Trypanosoma brucei
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Funding
- Swiss National Science Foundation (SNSF) [31003A-149353, PBBEP3_146277]
- SNSF Sinergia grant [CRSII3_141913]
- French National Research Agency [ANR-08-MIE-027]
- Ministere de la Recherche
- Fondation pour la Recherche Medicale [FDT20150532023]
- Swiss SystemsX.ch Initiative
- Swiss National Science Foundation (SNF) [31003A_149353, PBBEP3_146277, CRSII3_141913] Funding Source: Swiss National Science Foundation (SNF)
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The observation that the membranes of flagella are enriched in sterols and sphingolipids has led to the hypothesis that flagella might be enriched in raft-forming lipids. However, a detailed lipidomic analysis of flagellar membranes is not available. Novel protocols to detach and isolate intact flagella from Trypanosoma brucei procyclic forms in combination with reverse-phase liquid chromatography high-resolution tandem mass spectrometry allowed us to determine the phospholipid composition of flagellar membranes relative to whole cells. Our analyses revealed that phosphatidylethanolamine, phosphatidylserine, ceramide and the sphingolipids inositol phosphorylceramide and sphingomyelin are enriched in flagella relative to whole cells. In contrast, phosphatidylcholine and phosphatidylinositol are strongly depleted in flagella. Within individual glycerophospholipid classes, we observed a preference for ether-type over diacyl-type molecular species in membranes of flagella. Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei.
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