Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-017-04369-2
Keywords
-
Categories
Funding
- Singapore Ministry of Health's National Medical Research Council [NMRC/CSA/034/2012]
- National University of Singapore's Yong Loo Lin School of Medicine
- Medical Research Council (MRC)
- Metabolic Diseases Unit of the MRC
- British Heart foundation
- BBSRC [BB/J009865/1] Funding Source: UKRI
- MRC [G0400192, MC_UU_12012/2] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J009865/1] Funding Source: researchfish
- British Heart Foundation [PG/12/53/29714] Funding Source: researchfish
- Medical Research Council [MC_UU_12012/5/B, G0400192, MC_UU_12012/2] Funding Source: researchfish
Ask authors/readers for more resources
The Simpson Golabi Behmel Syndrome (SGBS) pre-adipocyte cell strain is widely considered to be a representative in vitro model of human white pre-adipocytes. A recent study suggested that SGBS adipocytes exhibit an unexpected transient brown phenotype. Here, we comprehensively examined key differences between SGBS adipocytes and primary human white subcutaneous (PHWSC) adipocytes. RNA-Seq analysis revealed that extracellular matrix (ECM)-receptor interaction and metabolic pathways were the top two KEGG pathways significantly enriched in SGBS adipocytes, which included positively enriched mitochondrial respiration and oxidation pathways. Compared to PHWSC adipocytes, SGBS adipocytes showed not only greater induction of adipogenic gene expression during differentiation but also increased levels of UCP1 mRNA and protein expression. Functionally, SGBS adipocytes displayed higher ISO-induced basal leak respiration and overall oxygen consumption rate, along with increased triglyceride accumulation and insulin-stimulated glucose uptake. In conclusion, we confirmed that SGBS adipocytes, which are considered of white adipose tissue origin can shift towards a brown/beige adipocyte phenotype. These differences indicate SGBS cells may help to identify mechanisms leading to browning, and inform our understanding for the use of SGBS vis-a-vis primary human subcutaneous adipocytes as a human white adipocyte model, guiding the selection of appropriate cell models in future metabolic research.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available