Journal
SCIENTIFIC REPORTS
Volume 7, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/srep40942
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Funding
- UK Department for the Environment, Food and Rural Affairs and devolved Scottish administration [SE0795]
- UK Department for the Environment, Food and Rural Affairs and devolved Welsh administration [SE2201]
- APHA Research and Development Internal Investment Fund Project [RD0092]
- Biotechnology and Biological Sciences Research Council [BBS/E/I/00002035] Funding Source: researchfish
- BBSRC [BBS/E/I/00002035] Funding Source: UKRI
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Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porcine blood, DC were enriched from PBMC by CD14 depletion and CD172a enrichment then stained with lineage mAbs (Lin; CD3, CD8 alpha, CD14 and CD21) and mAbs specific for CD172a, CD1 and CD4. Two distinct porcine cDC subpopulations were FACSorted CD1-cDC (Lin(-) CD172(+) CD1(-)CD4(-)) and CD1(+) cDC (Lin(-)CD172a(+) CD1(+) CD4(-)), and characterised by phenotypic and functional analyses. CD1(+) cDC were distinct from CD1-cDC, expressing higher levels of CD172a, MHC class II and CD11b. Following TLR stimulation, CD1(+) cDC produced IL-8 and IL-10 while CD1-cDC secreted IFN-alpha, IL-12 and TNF-alpha. CD1-cDC were superior in stimulating allogeneic T cell responses and in cross-presenting viral antigens to CD8 T cells. Comparison of transcriptional profiles further suggested that the CD1(-) and CD1(+) populations were enriched for the orthologues of cDC1 and cDC2 subsets respectively.
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