Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep38617
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Funding
- NSF of China [11374297, 61275202, 21304084, 11474278, 61575194, 11504371, 11604331, 11674316, 51372096]
- CAS of China
- KNAW of the Netherlands
- European union MSCA-ITN-ETN Action program
- ISPIC [675742]
- Netherlands Organisation for Scientific Research in the framework of the Fund New Chemical Innovation TA [731.015.206]
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Accurate quantitation of intracellular pH (pH(i)) is of great importance in revealing the cellular activities and early warning of diseases. A series of fluorescence-based nano-bioprobes composed of different nanoparticles or/and dye pairs have already been developed for pH(i) sensing. Till now, biological autofluorescence background upon UV-Vis excitation and severe photo-bleaching of dyes are the two main factors impeding the accurate quantitative detection of pHi. Herein, we have developed a selfratiometric luminescence nanoprobe based on frster resonant energy transfer (FRET) for probing pH(i), in which pH-sensitive fluorescein isothiocyanate (FITC) and upconversion nanoparticles (UCNPs) were served as energy acceptor and donor, respectively. Under 980 nm excitation, upconversion emission bands at 475 nm and 645 nm of NaYF4: Yb3+, Tm3+ UCNPs were used as pH(i) response and selfratiometric reference signal, respectively. This direct quantitative sensing approach has circumvented the traditional software-based subsequent processing of images which may lead to relatively large uncertainty of the results. Due to efficient FRET and fluorescence background free, a highly-sensitive and accurate sensing has been achieved, featured by 3.56 per unit change in pH(i) value 3.0-7.0 with deviation less than 0.43. This approach shall facilitate the researches in pH(i) related areas and development of the intracellular drug delivery systems.
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