Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep30640
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Funding
- National Science Foundation (NSF) Small Business Innovation Research (SBIR) Award Phase I from NSF IIP Division of Industrial Innovation and Partnerships [0945954]
- National Science Foundation (NSF) Small Business Innovation Research (SBIR) Award Phase II from NSF IIP Division of Industrial Innovation and Partnerships [1127551]
- Center for Advancement of Science in Space (CASIS)
- Directorate For Engineering
- Div Of Industrial Innovation & Partnersh [0945954] Funding Source: National Science Foundation
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Vasoactive liabilities are typically assayed using wire myography, which is limited by its high cost and low throughput. To meet the demand for higher throughput in vitro alternatives, this study introduces a magnetic 3D bioprinting-based vasoactivity assay. The principle behind this assay is the magnetic printing of vascular smooth muscle cells into 3D rings that functionally represent blood vessel segments, whose contraction can be altered by vasodilators and vasoconstrictors. A cost-effective imaging modality employing a mobile device is used to capture contraction with high throughput. The goal of this study was to validate ring contraction as a measure of vasoactivity, using a small panel of known vasoactive drugs. In vitro responses of the rings matched outcomes predicted by in vivo pharmacology, and were supported by immunohistochemistry. Altogether, this ring assay robustly models vasoactivity, which could meet the need for higher throughput in vitro alternatives.
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