Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep27290
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Funding
- Wellcome Trust Strategic Award [091911]
- MRC grant [MR/K01577X/1]
- Medical Research Council to the Molecular Haematology Unit [MC_UU_12009]
- Human Immunology Unit [MC_UU_12010]
- WIMM Strategic Alliance [G0902418, MC_UU_12025]
- Wolfson Foundation [18272]
- Wellcome Trust Multi-User Equipment grant [WT104924A1A]
- Sir Henry Wellcome Fellowship by the Wellcome Trust
- Junior Research Fellowship at St John's College, Oxford
- Medical Research Council [MR/K01577X/1] Funding Source: researchfish
- Wellcome Trust [107457/Z/15/Z] Funding Source: researchfish
- MRC [MR/K01577X/1] Funding Source: UKRI
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Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single Molecule Localisation Microscopy (SMLM) enable an increase in image resolution beyond the classical diffraction-limit. Here, we compare the individual strengths and weaknesses of each technique by imaging a variety of different subcellular structures in fixed cells. We chose examples ranging from well separated vesicles to densely packed three dimensional filaments. We used quantitative and correlative analyses to assess the performance of SIM, STED and SMLM with the aim of establishing a rough guideline regarding the suitability for typical applications and to highlight pitfalls associated with the different techniques.
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