Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/srep26317
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Funding
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/J01771X/1]
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Wellcome Trust
- BBSRC [BB/J018724/1] Funding Source: UKRI
- EPSRC [EP/J01771X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J018724/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/J01771X/1] Funding Source: researchfish
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Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45 degrees inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.
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