Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep24032
Keywords
-
Categories
Funding
- Collaborative Research Grant of the Berlin Institute of Health
- Einstein-Stiftung Berlin
- Berlin Krebsgesellschaft
- Belgian Program of Interuniversity Poles of Attraction
- Belgian State (Prime Minster's Office, Science Policy Programming)
Ask authors/readers for more resources
Proteasome-catalyzed peptide splicing represents an additional catalytic activity of proteasomes contributing to the pool of MHC-class I-presented epitopes. We here biochemically and functionally characterized a new melanoma gp100 derived spliced epitope. We demonstrate that the gp100(47-52/40-42)(mel) antigenic peptide is generated in vitro and in cellulo by a not yet described proteasomal condensation reaction. gp100(47-52/40-42)(mel) generation is enhanced in the presence of the beta 5i/LMP7 proteasome-subunit and elicits a peptide-specific CD8(+) T cell response. Importantly, we demonstrate that different gp100(mel)-derived spliced epitopes are generated and presented to CD8(+) T cells with efficacies comparable to non-spliced canonical tumor epitopes and that gp100(mel)-derived spliced epitopes trigger activation of CD8(+) T cells found in peripheral blood of half of the melanoma patients tested. Our data suggest that both transpeptidation and condensation reactions contribute to the frequent generation of spliced epitopes also in vivo and that their immune relevance may be comparable to non-spliced epitopes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available