Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep19550
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Funding
- National Natural Science Foundation of China [11372243, 11522219, 11532009]
- International Cooperation and Exchange Program of the National Natural Science Foundation of China [11120101002]
- International Science and Technology Cooperation Program of China [2013DFG02930]
- China Postdoctoral Science Foundation [2013M540742]
- Natural Science Basic Research Plan in Shaanxi Province of China [2014JQ1004]
- China Young 1000-Talent Program
- Changjiang (Yangtze River) Scholars Program of the Chinese Ministry of Education
- National Institutes of Health [R01HL109505]
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The tissues of hollow organs can routinely stretch up to 2.5 times their length. Although significant pathology can arise if relatively large stretches are sustained, the responses of cells are not known at these levels of sustained strain. A key challenge is presenting cells with a realistic and well-defined three-dimensional (3D) culture environment that can sustain such strains. Here, we describe an in vitro system called microscale, magnetically-actuated synthetic tissues (micro-MASTs) to quantify these responses for cells within a 3D hydrogel matrix. Cellular strain-threshold and saturation behaviors were observed in hydrogel matrix, including strain-dependent proliferation, spreading, polarization, and differentiation, and matrix adhesion retained at strains sufficient for apoptosis. More broadly, the system shows promise for defining and controlling the effects of mechanical environment upon a broad range of cells.
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