Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/srep21266
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Funding
- RIKEN Initiative Research Funding awarded
- RIKEN Junior Research Associate
- National Natural Science Foundation of China [31170672]
- Jiangsu Government Scholarship Fund [JS-2012-257]
- JSPS KAKENHI [23310088]
- Hong Kong University Grants Council under General Research Fund [HKU17119814, HKU778813M]
- Grants-in-Aid for Scientific Research [23310088, 24104004, 15H03541] Funding Source: KAKEN
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DNA aptamers have potential for disease diagnosis and as therapeutics, particularly when interfaced with programmable molecular technology. Here we have combined DNA aptamers specific for the malaria biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) with a DNA origami scaffold. Twelve aptamers that recognise PfLDH were integrated into a rectangular DNA origami and atomic force microscopy demonstrated that the incorporated aptamers preserve their ability to specifically bind target protein. Captured PfLDH retained enzymatic activity and protein-aptamer binding was observed dynamically using high-speed AFM. This work demonstrates the ability of DNA aptamers to recognise a malaria biomarker whilst being integrated within a supramolecular DNA scaffold, opening new possibilities for malaria diagnostic approaches based on DNA nanotechnology.
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