Interplay of histidine residues of the Alzheimer's disease Aβ peptide governs its Zn-induced oligomerization

Conformational changes of A beta peptide result in its transformation from native monomeric state to the toxic soluble dimers, oligomers and insoluble aggregates that are hallmarks of Alzheimer's disease (AD). Interactions of zinc ions with A beta are mediated by the N-terminal A beta(1-16) domain and appear to play a key role in AD progression. There is a range of results indicating that these interactions trigger the A beta plaque formation. We have determined structure and functional characteristics of the metal binding domains derived from several A beta variants and found that their zinc-induced oligomerization is governed by conformational changes in the minimal zinc binding site (6)HDSGYEVHH(14). The residue H6 and segment 11EVHH14, which are part of this site are crucial for formation of the two zinc-mediated interaction interfaces in A beta. These structural determinants can be considered as promising targets for rational design of the AD-modifying drugs aimed at blocking pathological A beta aggregation.