Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep21448
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Funding
- Karolinska Institutet (KID programme)
- Swedish Research Council
- Family Erling-Persson Foundation
- Novo Nordisk Foundation
- Stichting af Jochnick Foundation
- Swedish Diabetes Association
- Scandia Insurance Company Ltd.
- Diabetes Research and Wellness Foundation
- Berth von Kantzow's Foundation
- Strategic Research Program in Diabetes at Karolinska Institutet
- ERC-AdG [338936-BetaImage]
- Swedish Foundation for Strategic Research
- Knut and Alice Wallenberg Foundation
- JDRF award (ECIT Islet for Basic Research program) [31-2008-416]
- Novo Nordisk Fonden [NNF12OC1016557] Funding Source: researchfish
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Insulin resistance contributes to the development of cardio-vascular disease and diabetes. An important but unresolved task is to study the dynamics of insulin resistance in selective cell types of insulin target tissues in vivo. Here we present a novel technique to monitor insulin resistance dynamics non-invasively and longitudinally in vivo in a cell type-specific manner, exemplified by the pancreatic beta-cell situated within the micro-organ the islet of Langerhans. We utilize the anterior chamber of the eye (ACE) as a transplantation site and the cornea as a natural body-window to study the development and reversibility of insulin resistance. Engrafted islets in the ACE that express a FoxO1-GFP-based biosensor in their beta-cells, report on insulin resistance measured by fluorescence microscopy at single-cell resolution in the living mouse. This technique allows monitoring of cell type specific insulin sensitivity/resistance in real-time in the context of whole body insulin resistance during progression and intervention of disease.
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