Journal
SCIENTIFIC REPORTS
Volume 6, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep20350
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Funding
- Research Council Discovery Projects scheme [DP130100357]
- National Health and Medical Research Council
- Victorian Government's Operational Infrastructure
- Caenorhabditis Genetics Center of the US National Institutes of Health - Office of Research Infrastructure Programs [P40OD010440]
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We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (phi XANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.
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