Journal
SCIENTIFIC REPORTS
Volume 4, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep04272
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Funding
- National Basic Research Program of China (973 Program) [2013CB834701]
- Research Grants Council of Hong Kong [604711, 604913, HKUST2/CRF/10, N_HKUST620/11]
- University Grants Committee of Hong Kong [AoE/P-03/08]
- Guangdong Innovative Research Team Program of China [201101C0105067115]
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In this work, we design and synthesize a malonitrile-functionalized TPE derivative (TPE-DCV), which can react with thiol group through thiol-ene click reaction, leading to the fluorescence change of the system. Combined with the unique AIE property, TPE-DCV can selectively detect glutathione (GSH) but not cysteine or homocysteine. As the cleavage of GSSG with the aid of glutathione reductase produces GSH, which turns on the fluorescence of TPE-DCV, the ensemble of TPE-DCV and GSSG can thus serve as a label-free sensor for enzymatic activity assay of glutathione reductase. We also apply TPE-DCV for the detection of intracellular GSH in living cells.
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