Journal
SCIENTIFIC REPORTS
Volume 3, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep02176
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Funding
- National Science Foundation [CMMI 1129611, CBET 1149401]
- American Heart Association [12SDG12180025]
- National Institutes of Health [CA116592]
- department of Mechanical Engineering of the University of Michigan
- department of Biomedical Engineering of the University of Michigan
- National Science Foundation
- Directorate For Engineering [1129611, 1149401] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys [1149401] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn [1129611] Funding Source: National Science Foundation
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Mechanical forces are critical to modulate cell spreading, contractility, gene expression, and even stem cell differentiation. Yet, existing tools that can apply controllable subcellular forces to a large number of single cells simultaneously are still limited. Here we report a novel ultrasound tweezing cytometry utilizing ultrasound pulses to actuate functionalized lipid microbubbles covalently attached to single live cells to exert mechanical forces in the pN - nN range. Ultrasonic excitation of microbubbles could elicit a rapid and sustained reactive intracellular cytoskeleton contractile force increase in different adherent mechanosensitive cells. Further, ultrasound-mediated intracellular cytoskeleton contractility enhancement was dose-dependent and required an intact actin cytoskeleton as well as RhoA/ROCK signaling. Our results demonstrated the great potential of ultrasound tweezing cytometry technique using functionalized microbubbles as an actuatable, biocompatible, and multifunctional agent for biomechanical stimulations of cells.
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