Journal
SCIENTIFIC REPORTS
Volume 3, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep02490
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Funding
- Japan Society for the Promotion of Science [Kakenhi 23360135]
- Ministry of Education, Culture, Sports, Science & Technology (MEXT) [Kakenhi 20108016]
- Photon Frontier Network Program of MEXT, Japan
- International Priority Graduate Programs of MEXT, Japan
- Grants-in-Aid for Scientific Research [23360135] Funding Source: KAKEN
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Firefly bioluminescence has attracted great interest because of its high quantum yield and intriguing modifiable colours. Modifications to the structure of the enzyme luciferase can change the emission colour of firefly bioluminescence, and the mechanism of the colour change has been intensively studied by biochemists, structural biologists, optical physicists, and quantum-chemistry theorists. Here, we report on the quantitative spectra of firefly bioluminescence catalysed by wild-type and four site-directed mutant luciferases. While the mutation caused different emission spectra, the spectra differed only in the intensity of the green component (lambda(max) similar to 560 nm). In contrast, the orange (lambda(max) similar to 610 nm) and red (lambda(max) similar to 650 nm) components present in all the spectra were almost unaffected by the modifications to the luciferases and changes in pH. Our results reveal that the intensity of the green component is the unique factor that is influenced by the luciferase structure and other reaction conditions.
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