Journal
SCIENTIFIC REPORTS
Volume 1, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/srep00126
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- BBSRC
- Welcome Trust VIP
- Biotechnology and Biological Sciences Research Council [BB/C513369/1] Funding Source: researchfish
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A method is described for the detection of certain nucleotide modifications adjacent to the 5' 7-methyl guanosine cap of mRNAs from individual genes. The method quantitatively measures the relative abundance of 2'-O-methyl and N-6,2'-O-dimethyladenosine, two of the most common modifications. In order to identify and quantitatify the amounts of N-6,2'-O-dimethyladenosine, a novel method for the synthesis of modified adenosine phosphoramidites was developed. This method is a one step synthesis and the product can directly be used for the production of N-6,2'-O-dimethyladenosine containing RNA oligonucleotides. The nature of the cap-adjacent nucleotides were shown to be characteristic for mRNAs from individual genes transcribed in liver and testis.
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