4.6 Review

Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

Related references

Note: Only part of the references are listed.
Review Biochemistry & Molecular Biology

Engineering aldolases as biocatalysts

Claire L. Windle et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2014)

Article Biotechnology & Applied Microbiology

Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers

Jose C. S. dos Santos et al.

ENZYME AND MICROBIAL TECHNOLOGY (2014)

Article Biochemistry & Molecular Biology

Tuning of Lecitase features via solid-phase chemical modification: Effect of the immobilization protocol

Cristina Garcia-Galan et al.

PROCESS BIOCHEMISTRY (2014)

Article Biochemistry & Molecular Biology

Stabilizing hyperactivated lecitase structures through physical treatment with ionic polymers

Jose C. S. dos Santos et al.

PROCESS BIOCHEMISTRY (2014)

Review Biochemical Research Methods

Protein Engineering For Directed Immobilization

Erik Steen Redeker et al.

BIOCONJUGATE CHEMISTRY (2013)

Review Biochemistry & Molecular Biology

Polyionic and cysteine-containing fusion peptides as versatile protein tags

Hauke Lilie et al.

BIOLOGICAL CHEMISTRY (2013)

Review Chemistry, Multidisciplinary

Enzyme immobilisation in biocatalysis: why, what and how

Roger A. Sheldon et al.

CHEMICAL SOCIETY REVIEWS (2013)

Review Chemistry, Multidisciplinary

Modifying enzyme activity and selectivity by immobilization

Rafael C. Rodrigues et al.

CHEMICAL SOCIETY REVIEWS (2013)

Review Chemistry, Multidisciplinary

Stabilizing biocatalysts

Andreas S. Bommarius et al.

CHEMICAL SOCIETY REVIEWS (2013)

Article Biochemistry & Molecular Biology

Synthesis of ascorbyl oleate by transesterification of olive oil with ascorbic acid in polar organic media catalyzed by immobilized lipases

Sonia Moreno-Perez et al.

CHEMISTRY AND PHYSICS OF LIPIDS (2013)

Review Biotechnology & Applied Microbiology

Directed evolution: tailoring biocatalysts for industrial applications

Ashwani Kumar et al.

CRITICAL REVIEWS IN BIOTECHNOLOGY (2013)

Review Biochemistry & Molecular Biology

Strategies for the discovery and engineering of enzymes for biocatalysis

Timo Davids et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2013)

Article Biotechnology & Applied Microbiology

A general strategy for protein immobilization in layered titanates: Polyelectrolyte-assisted self-assembly

Zhu-Ping Han et al.

ENZYME AND MICROBIAL TECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

Stabilization of the hexameric glutamate dehydrogenase from Escherichia coli by cations and polyethyleneimine

Cristina Garcia-Galan et al.

ENZYME AND MICROBIAL TECHNOLOGY (2013)

Article Biochemical Research Methods

Purification of recombinant EGFP by fusion with L2 (252-273) from ribosomal protein L2 using magnetic particles

Junhua Li et al.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2013)

Article Chemistry, Multidisciplinary

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

Manfred T. Reetz

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Article Biochemistry & Molecular Biology

Enzymatic hydrolysis of racemic ibuprofen esters using Rhizomucor miehei lipase immobilized on different supports

Zohreh Habibi et al.

PROCESS BIOCHEMISTRY (2013)

Article Biochemistry & Molecular Biology

Oxidation of phenyl compounds using strongly stable immobilized-stabilized laccase from Trametes versicolor

Veria Addorisio et al.

PROCESS BIOCHEMISTRY (2013)

Review Chemistry, Multidisciplinary

Parameters in preparation and characterization of cross linked enzyme aggregates (CLEAs)

Sachin Talekar et al.

RSC ADVANCES (2013)

Review Chemistry, Multidisciplinary

Expanding the organic toolbox: a guide to integrating biocatalysis in synthesis

Christopher M. Clouthier et al.

CHEMICAL SOCIETY REVIEWS (2012)

Article Biochemical Research Methods

Comparison of magnetic carboxymethyl chitosan nanoparticles and cation exchange resin for the efficient purification of lysine-tagged small ubiquitin-like modifier protease

Junhua Li et al.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2012)

Article Biochemical Research Methods

Evaluation of chemically modified carrier proteins for developing monoclonal antibodies against a clinically relevant mutation of cKIT

Ricardo Jara-Acevedo et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2012)

Article Biochemistry & Molecular Biology

Preparation of cross-linked enzyme aggregates of L-aminoacylase via co-aggregation with polyethyleneimine

Bhalchandra K. Vaidya et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2012)

Article Biochemistry & Molecular Biology

Optimized preparation of CALB-CLEAs by response surface methodology: The necessity to employ a feeder to have an effective crosslinking

Jenniffer Cruz et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2012)

Article Chemistry, Applied

Modulation of the Selectivity of Immobilized Lipases by Chemical and Physical Modifications: Release of Omega-3 Fatty Acids from Fish Oil

Gloria Fernandez-Lorente et al.

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY (2012)

Article Biochemistry & Molecular Biology

Effect of solid-phase chemical modification on the features of the lipase from Thermomyces lanuginosus

Magaly Galvis et al.

PROCESS BIOCHEMISTRY (2012)

Review Chemistry, Applied

Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

Rafael C. Rodrigues et al.

ADVANCED SYNTHESIS & CATALYSIS (2011)

Review Chemistry, Applied

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

Cristina Garcia-Galan et al.

ADVANCED SYNTHESIS & CATALYSIS (2011)

Review Biotechnology & Applied Microbiology

Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)

Roger A. Sheldon

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2011)

Article Biotechnology & Applied Microbiology

Polycationic amino acid tags enhance soluble expression of Candida antarctica lipase B in recombinant Escherichia coli

Hyun-Jung Jung et al.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2011)

Article Engineering, Chemical

Activity, stability and inhibition of a bioactive paper prepared by large-scale coating of laccase microcapsules

M. P. Guerrero et al.

CHEMICAL ENGINEERING SCIENCE (2011)

Review Biochemical Research Methods

New opportunities for biocatalysis: driving the synthesis of chiral chemicals

Gao-Wei Zheng et al.

CURRENT OPINION IN BIOTECHNOLOGY (2011)

Review Biochemistry & Molecular Biology

Chemical modification in the creation of novel biocatalysts

Alba Diaz-Rodriguez et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2011)

Review Biotechnology & Applied Microbiology

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

Don A. Cowan et al.

ENZYME AND MICROBIAL TECHNOLOGY (2011)

Review Biotechnology & Applied Microbiology

Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

Karel Hernandez et al.

ENZYME AND MICROBIAL TECHNOLOGY (2011)

Article Biochemistry & Molecular Biology

Electrochemically synthesized polyaniline as support for lipase immobilization

Dejan Bezbradica et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2011)

Article Biochemistry & Molecular Biology

Hen egg white as a feeder protein for lipase immobilization

Joceline J. Karimpil et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2011)

Article Electrochemistry

Oriented immobilization and electron transfer to the cytochrome c oxidase

Christoph Nowak et al.

JOURNAL OF SOLID STATE ELECTROCHEMISTRY (2011)

Article Biochemical Research Methods

Production and solid-phase refolding of human glucagon-like peptide-1 using recombinant Escherichia coli

Sung-Gun Kim et al.

PROTEIN EXPRESSION AND PURIFICATION (2011)

Article Chemistry, Physical

Engineering of Biocatalysts: from Evolution to Creation

Maureen B. Quin et al.

ACS CATALYSIS (2011)

Review Biophysics

Immobilization of biomolecules on nanostructured films for biosensing

Jose R. Siqueira et al.

BIOSENSORS & BIOELECTRONICS (2010)

Article Chemistry, Physical

Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine

Zaida Cabrera et al.

CATALYSIS COMMUNICATIONS (2010)

Review Biochemistry & Molecular Biology

Protein Chemical Modification on Endogenous Amino Acids

Emmanuel Basle et al.

CHEMISTRY & BIOLOGY (2010)

Review Biochemistry & Molecular Biology

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

G. Volpato et al.

CURRENT MEDICINAL CHEMISTRY (2010)

Article Biochemistry & Molecular Biology

Polyethyleneimine-protein interactions and implications on protein stability

Laura Mazzaferro et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2010)

Article Biotechnology & Applied Microbiology

Preparation of carboxylated magnetic particles for the efficient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II

Cheng-Liang Huang et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2010)

Article Chemistry, Physical

Vinyl sulfone functionalized silica: a ready to use'' pre-activated material for immobilization of biomolecules

Mariano Ortega-Munoz et al.

JOURNAL OF MATERIALS CHEMISTRY (2010)

Article Chemistry, Physical

Preparation of dichlorvos-protein complete antigen by Mannich-type reaction

Qianqian Feng et al.

JOURNAL OF MOLECULAR STRUCTURE (2010)

Article Chemistry, Organic

Vinyl sulfone: a versatile function for simple bioconjugation and immobilization

Julia Morales-Sanfrutos et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2010)

Article Biochemistry & Molecular Biology

Enhanced activity of an immobilized lipase promoted by site-directed chemical modification with polymers

Cesar A. Godoy et al.

PROCESS BIOCHEMISTRY (2010)

Review Biotechnology & Applied Microbiology

Advances in enzyme immobilisation

Dean Brady et al.

BIOTECHNOLOGY LETTERS (2009)

Review Chemistry, Multidisciplinary

Selective Covalent Protein Immobilization: Strategies and Applications

Lu Shin Wong et al.

CHEMICAL REVIEWS (2009)

Review Chemistry, Multidisciplinary

Chemical Modification of Proteins at Cysteine: Opportunities in Chemistry and Biology

Justin M. Chalker et al.

CHEMISTRY-AN ASIAN JOURNAL (2009)

Review Biotechnology & Applied Microbiology

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

Roberto Fernandez-Lafuente

ENZYME AND MICROBIAL TECHNOLOGY (2009)

Article Biochemistry & Molecular Biology

Activation of Bacterial Thermoalkalophilic Lipases Is Spurred by Dramatic Structural Rearrangements

Cesar Carrasco-Lopez et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2009)

Article Biochemical Research Methods

Preparation and characterization of bisphenol A-cationized bovine serum albumin

Yi Feng et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2009)

Article Biochemistry & Molecular Biology

Immobilization-stabilization of the lipase from Thermomyces lanuginosus: Critical role of chemical amination

Rafael C. Rodrigues et al.

PROCESS BIOCHEMISTRY (2009)

Article Biochemistry & Molecular Biology

Enhancement of Novozym-435 catalytic properties by physical or chemical modification

Zaida Cabrera et al.

PROCESS BIOCHEMISTRY (2009)

Article Biochemistry & Molecular Biology

Kinetic and Thermodynamic Study of a Chemically Modified Highly Active Xylanase from Scopulariopsis sp.

Ahmed Jawaad Afzal et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2008)

Article Biotechnology & Applied Microbiology

Immobilization-stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus

Juan M. Bolivar et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2008)

Article Biotechnology & Applied Microbiology

Practical application of different enzymes immobilized on sepabeads

L. Hilterhaus et al.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2008)

Review Biochemistry & Molecular Biology

Design of cytotoxic ribonucleases by cationization to enhance intracellular protein delivery

Junichiro Futami et al.

CURRENT PHARMACEUTICAL BIOTECHNOLOGY (2008)

Review Electrochemistry

Gold nanoparticle-based electrochemical biosensors

Jose M. Pingarron et al.

ELECTROCHIMICA ACTA (2008)

Review Biotechnology & Applied Microbiology

The realm of penicillin G acylase in β-lactam antibiotics

Anuj K. Chandel et al.

ENZYME AND MICROBIAL TECHNOLOGY (2008)

Article Biotechnology & Applied Microbiology

Transient cell proliferation with polyethylenimine-cationized N-terminal domain of simian virus 40 large T-antigen

Hitoshi Murata et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2008)

Article Biochemistry & Molecular Biology

Immobilization of invertase-chitosan conjugate on hyaluronic-acid-modified chitin

Leissy Gomez et al.

JOURNAL OF FOOD BIOCHEMISTRY (2008)

Article Biochemistry & Molecular Biology

Characterization of lysine-tagged Bacillus stearothermophilus leucine aminopeptidase II immobilized onto carboxylated gold nanoparticles

Chien-Li Wu et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2008)

Review Biochemistry & Molecular Biology

Enzyme stability and stabilization - Aqueous and non-aqueous environment

Padma V. Iyer et al.

PROCESS BIOCHEMISTRY (2008)

Review Biochemistry & Molecular Biology

Recent developments in the site-specific immobilization of proteins onto solid supports

Julio A. Camarero

BIOPOLYMERS (2008)

Article Biochemistry & Molecular Biology

Advances in the design of new epoxy supports for enzyme immobilization-stabilization

C. Mateo et al.

BIOCHEMICAL SOCIETY TRANSACTIONS (2007)

Article Biochemical Research Methods

Preparation for aflatoxin B1-cationized bovine serum albumin based on Mannich-type reaction

Youxiang Zhou et al.

JOURNAL OF IMMUNOLOGICAL METHODS (2007)

Article Biotechnology & Applied Microbiology

New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase

Davide A. Cecchini et al.

BMC BIOTECHNOLOGY (2007)

Article Chemistry, Multidisciplinary

Layered titanate nanosheets intercalated with myoglobin for direct electrochemistry

Ling Zhang et al.

ADVANCED FUNCTIONAL MATERIALS (2007)

Review Biotechnology & Applied Microbiology

Improvement of enzyme activity, stability and selectivity via immobilization techniques

Cesar Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2007)

Article Chemistry, Applied

Modulation of immobilized lipase enantioselectivity via chemical amination

Jose M. Palomo et al.

ADVANCED SYNTHESIS & CATALYSIS (2007)

Article Biochemical Research Methods

Solid phase proteomics:: Dramatic reinforcement of very weak protein-protein interactions

Manuel Fuentes et al.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2007)

Article Biochemistry & Molecular Biology

Chemical modification results in hyperactivation and thermostabilization of Fusarium solani glucoamylase

Haq Nawaz Hatti et al.

CANADIAN JOURNAL OF MICROBIOLOGY (2007)

Review Pharmacology & Pharmacy

Exploiting protein cationization techniques in future drug development

Junichiro Futami et al.

EXPERT OPINION ON DRUG DISCOVERY (2007)

Article Biotechnology & Applied Microbiology

Genetic modification of the penicillin G acylase surface to improve its reversible immobilization on ionic exchangers

Tamara Montes et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2007)

Article Biochemistry & Molecular Biology

Chemical modification of protein surfaces to improve their reversible enzyme immobilization on ionic exchangers

Tamara Montes et al.

BIOMACROMOLECULES (2006)

Review Biotechnology & Applied Microbiology

Natural and engineered ribonucleases as potential cancer therapeutics

Ulrich Arnold et al.

BIOTECHNOLOGY LETTERS (2006)

Article Biochemistry & Molecular Biology

Glutaraldehyde cross-linking of lipases adsorbed on aminated supports in the presence of detergents leads to improved performance

Gloria Fernandez-Lorente et al.

BIOMACROMOLECULES (2006)

Article Biotechnology & Applied Microbiology

CLEAs of lipases and poly-ionic polymers:: A simple way of preparing stable biocatalysts with improved properties

Lorena Wilson et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Article Biotechnology & Applied Microbiology

Glyoxyl agarose:: A fully inert and hydrophilic support for immobilization and high stabilization of proteins

Cesar Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Article Biotechnology & Applied Microbiology

Transglutaminase-catalysed glycosidation of trypsin with aminated polysaccharides

ML Villalonga et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2006)

Article Biotechnology & Applied Microbiology

Glyoxyl agarose as a new chromatographic matrix

V Grazu et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Article Biotechnology & Applied Microbiology

Polyelectrolyte complex formation mediated immobilization of chitosan-invertase neoglycoconjugate on pectin-coated chitin

Leissy Gomez et al.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2006)

Article Biochemical Research Methods

Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder

S Shah et al.

ANALYTICAL BIOCHEMISTRY (2006)

Article Biochemistry & Molecular Biology

Stabilisation of oxygen-labile nitrilases via co-aggregation with poly (ethyleneimine)

C Mateo et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2006)

Article Chemistry, Multidisciplinary

Liver targeting of catalase by cationization for prevention of acute liver failure in mice

SF Ma et al.

JOURNAL OF CONTROLLED RELEASE (2006)

Article Biotechnology & Applied Microbiology

Immobilization of chitosan-modified invertase on alginate-coated chitin support via polyelectrolyte complex formation

L Gómez et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Article Biochemistry & Molecular Biology

Polyarginine as a multifunctional fusion tag

SM Fuchs et al.

PROTEIN SCIENCE (2005)

Article Biotechnology & Applied Microbiology

Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag

F Scaramozzino et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2005)

Review Engineering, Chemical

Protein engineering strategies for selective protein purification

M Hedhammar et al.

CHEMICAL ENGINEERING & TECHNOLOGY (2005)

Article Biotechnology & Applied Microbiology

Some special features of glyoxyl supports to immobilize proteins

C Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2005)

Article Biochemistry & Molecular Biology

Production of cyclodextrin by poly-lysine fused Bacillus macerans cyclodextrin glycosyltransferase immobilized on cation exchanger

CS Rha et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2005)

Review Biotechnology & Applied Microbiology

Making the most of affinity tags

DS Waugh

TRENDS IN BIOTECHNOLOGY (2005)

Article Biochemistry & Molecular Biology

Protein transduction assisted by polyethylenimine-cationized carrier proteins

M Kitazoe et al.

JOURNAL OF BIOCHEMISTRY (2005)

Article Biotechnology & Applied Microbiology

Immobilization of Bacillus macerans cyclodextrin glycosyltransferase fused with poly-lysine using cation exchanger

DH Kweon et al.

ENZYME AND MICROBIAL TECHNOLOGY (2005)

Article Biotechnology & Applied Microbiology

Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports

F López-Gallego et al.

JOURNAL OF BIOTECHNOLOGY (2005)

Article Chemistry, Multidisciplinary

Cationic charge-dependent hepatic delivery of amidated serum albumin

SF Ma et al.

JOURNAL OF CONTROLLED RELEASE (2005)

Article Biotechnology & Applied Microbiology

Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization

J Futami et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2005)

Article Biochemistry & Molecular Biology

Dextran aldehyde coating of glucose oxidase immobilized on magnetic nanoparticles prevents its inactivation by gas bubbles

L Betancor et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2005)

Article Biochemical Research Methods

Ion exchange using poorly activated supports, an easy way for purification of large proteins

BCC Pessela et al.

JOURNAL OF CHROMATOGRAPHY A (2004)

Article Biochemistry & Molecular Biology

Cytotoxicity of RNases is increased by cationization and counteracted by KCa channels

ON Ilinskaya et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2004)

Article Biochemistry & Molecular Biology

Immobilization of rennet from Mucor miehei via its sugar chain.: Its use in milk coagulation

BCC Pessela et al.

BIOMACROMOLECULES (2004)

Article Biotechnology & Applied Microbiology

Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate-dextran

M Fuentes et al.

BIOTECHNOLOGY PROGRESS (2004)

Article Chemistry, Multidisciplinary

Enhanced catalytic activity of hemoglobin in organic solvents by layered titanate immobilization

QG Wang et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)

Article Biotechnology & Applied Microbiology

Prevention of interfacial inactivation of enzymes by coating the enzyme surface with dextran-aldehyde

L Betancor et al.

JOURNAL OF BIOTECHNOLOGY (2004)

Review Biotechnology & Applied Microbiology

Enzyme stabilization -: recent experimental progress

C O'Fágáin

ENZYME AND MICROBIAL TECHNOLOGY (2003)

Review Biochemical Research Methods

Chemical modification of biocatalysts

BG Davis

CURRENT OPINION IN BIOTECHNOLOGY (2003)

Article Biotechnology & Applied Microbiology

Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties

G Fernández-Lorente et al.

BIOTECHNOLOGY AND BIOENGINEERING (2003)

Article Multidisciplinary Sciences

Dispelling the myths - Biocatalysis in industrial synthesis

HE Schoemaker et al.

SCIENCE (2003)

Article Biotechnology & Applied Microbiology

Effects of phthalic anhydride modification on horseradish peroxidase stability and activity

AM O'Brien et al.

BIOTECHNOLOGY AND BIOENGINEERING (2003)

Article Biochemistry & Molecular Biology

Changing the net charge from negative to positive makes ribonuclease Sa cytotoxic

ON Ilinskaya et al.

PROTEIN SCIENCE (2002)

Article Biochemistry & Molecular Biology

Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-Å resolution containing a full complement of coppers

K Piontek et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2002)

Article Biochemistry & Molecular Biology

Optimum modification for the highest cytotoxicity of cationized ribonuclease

J Futami et al.

JOURNAL OF BIOCHEMISTRY (2002)

Review Pharmacology & Pharmacy

Chemistry for peptide and protein PEGylation

MJ Roberts et al.

ADVANCED DRUG DELIVERY REVIEWS (2002)

Article Biochemistry & Molecular Biology

The role of poly(ethyleneimine) in stabilization against metal-catalyzed oxidation of proteins: a case study with lactate dehydrogenase

JD Breccia et al.

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2002)

Article Biotechnology & Applied Microbiology

Effects of charged water-soluble polymers on the stability and activity of yeast alcohol dehydrogenase and subtilisin Carlsberg

TM Foreman et al.

BIOTECHNOLOGY AND BIOENGINEERING (2001)

Article Biochemistry & Molecular Biology

Crystal structures of penicillin acylase enzyme-substrate complexes: Structural insights into the catalytic mechanism

CE McVey et al.

JOURNAL OF MOLECULAR BIOLOGY (2001)

Article Biochemistry & Molecular Biology

Stability and stabilization of hydroxynitrile lyase in organic solvents

D Costes et al.

BIOCATALYSIS AND BIOTRANSFORMATION (2001)

Article Biochemistry & Molecular Biology

Structural origins of the interfacial activation in Thermomyces (Humicola) lanuginosa lipase

AM Brzozowski et al.

BIOCHEMISTRY (2000)

Article Chemistry, Multidisciplinary

Modulation of electroenzymatic NADPH oxidation through oriented immobilization of ferredoxin:NADP+ reductase onto modified gold electrodes

J Madoz-Gúrpide et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2000)

Article Biochemistry & Molecular Biology

Charge engineering of a protein domain to allow efficient ion-exchange recovery

T Gräslund et al.

PROTEIN ENGINEERING (2000)

Article Biochemistry & Molecular Biology

Eupergit® C, a carrier for immobilization of enzymes of industrial potential

E Katchalski-Katzir et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2000)

Article Biotechnology & Applied Microbiology

Functional stabilization of invertase by covalent modification with pectin

L Gómez et al.

BIOTECHNOLOGY LETTERS (2000)

Article Biochemical Research Methods

Polymer displacement/shielding in protein chromatography

A Kumar et al.

JOURNAL OF CHROMATOGRAPHY B (2000)

Article Biotechnology & Applied Microbiology

Stabilization of invertase by modification of sugar chains with chitosan

L Gomez et al.

BIOTECHNOLOGY LETTERS (2000)

Article Biochemistry & Molecular Biology

Stabilisation of chloroperoxidase towards peroxide dependent inactivation

M Andersson et al.

BIOCATALYSIS AND BIOTRANSFORMATION (2000)