Journal
RSC ADVANCES
Volume 4, Issue 3, Pages 1334-1340Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ra44717k
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Funding
- NIH [R01GM105686]
- Arnold and Mabel Beckman Foundation
- Camille and Henry Dreyfus Foundation
- 3M
- Pennsylvania State University
- Alfred P. Sloan Research Fellows program
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM105686] Funding Source: NIH RePORTER
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Fluorescence assays often require specialized equipment and, therefore, are not easily implemented in resource-limited environments. Herein we describe a point-of-care assay strategy in which fluorescence in the visible region is used as a readout, while a camera-equipped cellular phone is used to capture the fluorescent response and quantify the assay. The fluorescence assay is made possible using a paper-based microfluidic device that contains an internal fluidic battery, a surface-mount LED, a 2 mm section of a clear straw as a cuvette, and an appropriately designed small molecule reagent that transforms from weakly fluorescent to highly fluorescent when exposed to a specific enzyme biomarker. The resulting visible fluorescence is digitized by photographing the assay region using a camera-equipped cellular phone. The digital images are then quantified using image processing software to provide sensitive as well as quantitative results. In a model 30 min assay, the enzyme beta-D-galactosidase was measured quantitatively down to 700 pM levels. This communication describes the design of these types of assays in paper-based microfluidic devices and characterizes the key parameters that affect the sensitivity and reproducibility of the technique.
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