Journal
RSC ADVANCES
Volume 4, Issue 43, Pages 22817-22827Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra01428f
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In the present study, a marine sponge-associated endosymbiotic bacterium Bacillus megaterium MSBN04 was evaluated for exopolysaccharide (EPS) production. The production process was optimized by central composite design (CCD). The productivity was increased up to 5.62 g L-1 with sucrose as sole carbon source. The secreted EPS was characterized by NMR analysis, confirming the presence of monosaccharide units such as alpha-D-glucose and alpha-D-galactose, which further confirms that the secreted EPS is a heteropolysaccharide. The purified EPS showed considerable flocculating activity (45.41%) with 4 mg L-1 of EPS. Using EPS as reducing and stabilizing agent, gold nanoparticles (AuNPs) were synthesized. The synthesized AuNPs (5-20 nm) were of spherical crystalline nature and capped with an EPS layer and were characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD) analysis. The synthesis of AuNPs was dependent on the concentration of EPS. The synthesized AuNPs showed significant antibacterial activity against clinical pathogenic bacteria. Hence, EPS-mediated synthesis of AuNPs is an alternative approach to chemical synthesis and thus it is an environmentally benign, greener and economical approach.
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