Journal
RSC ADVANCES
Volume 4, Issue 97, Pages 54650-54654Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra09712b
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Funding
- National Basic Research Program of China [2010CB933504]
- National Natural Science Foundation of China [91313303]
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We designed and synthesized a two-photon fluorescent probe, 2-(1,5-diphenyl-4,5-dihydro-1H-pyrazol-3-yl)naphthalen-1-yl acrylate (PPN) with improved properties based on the naphthalene-pyrazoline fluorophore. The probe exhibits high sensitivity to glutathione (GSH) in PBS-CTAB buffer solution with a low detection limit of 1.5 x 10(-8) M and a response time less than 10 min. According to the HRMS and fluorescence spectra analysis, the detection mechanism was confirmed to be a Michael addition reaction induced by the sulfhydryl of GSH. In addition, probe PPN has very good selectivity and is able to discriminate GSH from cysteine (Cys), homocysteine (Hcy) and other sulfhydryl compounds with bright two-photon-excited fluorescence. Moreover, we have successfully applied PPN to calf serum samples and living cell imaging with good effect.
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