Journal
RSC ADVANCES
Volume 4, Issue 100, Pages 56809-56815Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra10311d
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Funding
- VI National RDi Plan
- Iniciativa Ingenio
- Consolider Program
- CIBER Actions
- Instituto de Salud Carlos III
- European Regional Development Fund
- Commission for Universities and Research of the Department of Innovation, Universities, and Enterprise of the Generalitat de Catalunya [2009 SGR 505]
- Fundacion M. Botin, Santander, Spain
- European Community
- European Research Infrastructure EUMINAfab - FP7 specific program Capacities [226460]
- Karlsruhe Nano Micro Facility (KNMF)
- Helmholtz Research Infrastructure at Karlsruhe Institute of Technology (KIT)
- CICYT [CTQ2012-30930]
- Generalitat of Catalunya [2009SGR1024]
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Dip-pen nanolithography and microcontact printing were used to fabricate mesopatterned substrates for cell differentiation experiments. A biotin-thiol was patterned on gold substrates and subsequently functionalised with streptavidin and biotinylated bone morphogenetic protein-2 (BMP-2). The feasibility of mesopatterned substrates containing immobilised BMP-2 was proven by obtaining similar differentiation outcomes compared to the growth factor in solution. Therefore, these substrates might be suitable for replacing conventional experiments with BMP-2 in solution.
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