Journal
RSC ADVANCES
Volume 4, Issue 97, Pages 54872-54878Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra09603g
Keywords
-
Categories
Funding
- Fundacao para a Ciencia e a Tecnologia (FCT, Lisbon)
- Projecto de Re-equipamento Cientifico, Portugal
- FCT [PEst-C/CTM/LA0025/2013, PEst-OE/SAU/UI0009/2013, PEst-C/EQB/LA0006/2013, PTDC/EQU-EQU/116097/2009, PTDC/BBB-NAN/1812/2012, PTDC/CTM/099452/2008, SFRH/BD/66858/2009, SFRH/BD/62957/2009]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/66858/2009, SFRH/BD/62957/2009, PEst-C/CTM/LA0025/2013, PTDC/BBB-NAN/1812/2012, PTDC/EQU-EQU/116097/2009, PTDC/CTM/099452/2008] Funding Source: FCT
Ask authors/readers for more resources
The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are 'smart' biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available