Journal
RSC ADVANCES
Volume 4, Issue 91, Pages 50097-50101Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ra09066g
Keywords
-
Categories
Funding
- National Science Foundation of China [21001013]
Ask authors/readers for more resources
In this paper, we developed a novel fluorescent probe C359 for highly selective detection of H2S over other relevant biothiols. C359 is designed to contain a thiol-specific cleavable disulfide bond. H2S-mediated the disulfide cleavage and subsequent intramolecular cyclization released the masked 7-hydroxyl coumarin, displaying a remarkable fluorescence enhancement. With the promising features in hand, C359 has been applied to detect the activity of CSE (one of H2S-producing enzyme) and build up an assay for screening CSE inhibitors. We anticipated that the enzyme assay using C359 could provide a powerful methodology for screening more potent and selective enzyme inhibitors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available